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KMID : 0364820120480040319
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Korean Journal of Microbiology
2012 Volume.48 No. 4 p.319 ~ p.324
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Molecular Divergences of 16S rRNA and rpoB Gene in Marine Isolates of the Order Oscillatoriales (Cyanobacteria)
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Cheon Ju-Yong
Lee Min-Ah
Ki Jang-Seu
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Abstract
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In this study, we investigated molecular divergences and phylogenetic characteristics of the 16S ribosomal RNA
(rRNA) and RNA polymerase beta subunit (rpoB) gene sequences from the order Oscillatoriales (Cyanobacteria).
The rpoB of Oscillatoriales showed higher genetic divergence when compared with those of 16S rRNA (p-distance:
rpoB=0.270, 16S=0.109), and these differences were statistically significant (Student t-test, p<0.001). Phylogenetic
trees of 16S rRNA and rpoB were generally compatible; however, rpoB tree clearly separated the compared
Oscillatoriales taxa, with higher phylogenetic resolution. In addition, parsimony analyses showed that rpoB gene
evolved 2.40-fold faster than 16S rRNA. These results suggest that the rpoB is a useful gene for the molecular
phylogenetics and species discrimination in the order Oscillatoriales.
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KEYWORD
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Oscillatoriales, rpoB gene, Cyanobacteria, DNA similarity, genetic distance
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